RTT Releases HIV-1 Integrase LDT Assay

Despite continuing advances in treatment for HIV‐1 infection, antiretroviral therapy (ART) may fail due to the accumulation of drug resistance mutations or the transmission of drug resistance mutations pre‐therapy. Identifying such mutations can optimize and guide selection of antiretroviral drugs. Thus, detection of mutations associated with antiviral resistance is recommended at entry into care, when ART is initiated or changed, when treatment failure is suspected, or when otherwise clinically indicated. When virologic failure is suspected, resistance testing should be performed while the patient is taking the drug or within 3 weeks after discontinuing it.

This HIV‐1 Integrase Genotype test is designed to detect mutations associated with resistance to integrase class of inhibitors (INI) also known as HIV integrase strand transfer inhibitor (INSTI). These INI’s represent a new antiretroviral drug class in which the catalytic activity of integrase enzyme is inhibited, thereby preventing formation of the HIV provirus required for viral propagation and integration into host chromosome. The major mutations associated with raltegravir (RTG) resistance include N155H, Q148H/K/R, and Y143C/H/R.3,4 and for elvitegravir (EVG) resistance mutations include T66A/K/I, E92Q/G, T97A, S147G, Q148H/K/R, N155H5,6 (**Note cross‐resistant profiles of INI6,,7,8) and the mutations E92Q, L101I, T124A, Q148H/R, and S153Y/F7,8,9 for dolutegravir, the experimental expanded access second generation INI. In addition, minor mutations that may contribute to drug resistance as compensatory, Resistance Associated Mutations (RAM), are reported, as well as non‐RAM polymorphisms (mutations that do not have reported resistance effects) which make up the virus “Polymorphic Fingerprint”.

For more information, please contact Robert Lloyd @ lloyd@researchthinktank.com.

Posted in Assay Release | Leave a comment

Welcome to the New Research Think Tank, Inc!

In the past year, there has been a lot going on with Research Think Tank, Inc. (RTT) Between new products, expanded service lines, great research projects and amazing presentations at some of the most important scientific meetings around the world, we have a lot to offer you in the coming year.

Additionally, as you can see there’s a lot going on with our website right now. We have a brand new design that will allow us to deploy more information and service announcements as well as communicate with you, our client, much better. We urge you to sign-up for our newsletter to get those latest announcements as soon as they come out. We’ll also be lettering you know about events both here at RTT as well as abroad at scientific meetings and trade shows.

You can also subscribe to the RSS feed on our blog, which will update when we post new items. So, enjoy the new site, stay a while, browse around and feel free to share the new RTT with your friends and colleagues!

Posted in Uncategorized | Leave a comment